RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Bovine,Canine,Guineapig,Hamster,Human,Mouse,Non-humanprimate,Rabbit,Rat,Sheep
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~46kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p145-153,RRID:AB_2632396
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoTyr153ofhumanCK1Mt.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Creatinekinase(CK1)playsanimportantroletransferringphosphategroupsfromphosphocreatinetoADPinthecytosoloftissueshavinghigh,fluctuatingenergydemandslikeskeletalmuscle,heart,andbrain. MitochondrialCK1,CK1Mt,hastwoisoforms,sarcomericCK1MtandubiquitousCK1Mt. CK1MtislocalizedbetweentheMIM(mitochondrialinnermembrane)andMOM(mitochondrialoutermembrane)andboundtothecardiolipin-richinnerleaflet(Mulleretal.,1985)andalongthecristaemembranes(Wegmannetal.,1991). CK1Mtexistsintwoforms;ahomodimerandanoctamerconsistingoffourhomodimers. TheCK1MtoctamerformspermanentcontactsitesandmaintainsacomplexstructureincludingporinandANT(adeninenucleotidetranslocase)withinMIMandMOM(Speeretal,2005).Therehavebeenseveralphospho-serine,threonine,andtyrosinesitesidentifiedwithinCK1Mt,theroleofeachonehasyettobedetermined.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Müller,M.,Moser,R.,Cheneval,D.,Carafoli,E.(1985)CardiolipinistheMembraneReceptorforMitochondrialCreatinePhosphokinase.J.Biol.Chem.260,3839-3843.
Wegmann,G.,Huber,R.,Zanolla,E.,Eppenberger,H.M.,Wallimann,T.(1991)DifferentialExpressionandLocalizationofBrain-TypeandMitochondrialCreatineKinaseIsoenzymesDuringDevelopmentoftheChickenRetina:Mi-CKasaMarkerforDifferentiationofPhotoreceptorCells.Differentiation46,77-87.
SpeerO,BäckN,BuerklenT,BrdiczkaD,KoretskyA,WallimanT,ErikssonO(2005)OctamericMitochondrialCreatineKinaseInducesandStABIlizesContactSitesBetweentheInnerandOuterMembrane.Biochem.J.385,445-450.
