Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IF1:100
- Species:
- Mouse
- MolecularReference:
- ~58kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#597-FTO,RRID:AB_2492098
Antigen/Purification:Collapse
TheantigenissyntheticpeptidefromtheN-terminalregionofratFTO.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
TheFTOgeneisthemostrobustgeneforcommonobesitycharacterizedtodate.FTOgeneexpressionhasbeenfoundtobesignificantlyupregulatedinthehypothalamusofratsafterfooddeprivationandstronglynegativelycorrelatedwiththeexpressionoforexinpeptidewhichisinvolvedinthestimulationoffoodintake(FredrickssonRetal.,2008).DeletionanalysisofFTOgeneinmiceshowedthatFtoisfunctionallyinvolvedinthecontrolofbothenergyintakeandenergyexpenditure(FischerJetal.,2009).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforuptooneyearafterdateofreceipt.Avoidfreeze/thawcycles.
ProductSpecificReferences
Guan,B.J.,vanHoef,V.,Jobava,R.,Elroy-Stein,O.,Valasek,L.S.,Cargnello,M.,Gao,X.H.,Krokowski,D.,Merrick,W.C.,Kimball,S.R.andKomar,A.A.,2017.AUniqueISRProgramDeterminesCellularResponsestoChronicStress.MolecularCell,68(5),pp.885-900.PMID:29220654
Yu,J.,Chen,M.,Huang,H.,Zhu,J.,Song,H.,Zhu,J.,Park,J.andJi,S.J.,2017.Dynamicm6AmodificationregulateslocaltranslationofmRNAinaxons.NucleicAcidsResearch.Nov23.PMID:29186567
Zhou,J.,Wan,J.,Gao,X.,Zhang,X.,Jaffrey,S.R.,&Qian,S.B.(2015).Dynamicm6AmRNAmethylationdirectstranslationalcontrolofheatshockresponse.Nature,526(7574),591-594.PMID:26458103
Gulati,P.,Avezov,E.,Ma,M.,Antrobus,R.,Lehner,P.,O’Rahilly,S.,&Yeo,G.S.(2014).Fatmassandobesity-related(FTO)shuttlesbetweenthenucleusandcytoplasm.Biosciencereports,34(5),621-628.PMID:25242086
GulatiP,CheungMK,AntrobusR,ChurchCD,HardingHP,TungYC,RimmingtonD,MaM,RonD,LehnerPJ,AshcroftFM,CoxRD,CollAP,O’RahillyS,YeoGS(2013)Rolefortheobesity-relatedFTOgeneinthecellularsensingofaminoacids.ProcNatlAcadSciUSA.Jan28.PMID:23359686
