Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- PPP1R1B
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1025-137,RRID:AB_2492070
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser137ofratDARPP-32.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
DARPP-32isadopamine(DA)andcAMP-regulated~32kDaphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).Serine137isphosphorylatedbycaseinkinase1andphosphorylationofthissiteisincreasedfollowingacuteadmiNISTrationofProzac®(Svenningssonetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BibbJA,SnyderGL,NishiA,YanZ,MeijerL,FienbergAA,TsaiLH,KwonYT,GiraultJA,CzernikAJ,HuganirRL,HemmingsHC,Jr.,NairnAC,GreengardP(1999)PhosphorylationofDARPP-32bycdk5modulatesdopaminesignallinginneurons.Nature(London)402:669-671.
Fienberg,A.A.,Hiroi,N.,Mermelstein,P.G.,Song,W.,Snyder,G.L.,Nishi,A.,Cheramy,A.O’Callaghan,J.P.,Miller,D.B.,Cole,D.G.,Corbett,R.,Haile,C.N.,Cooper,D,C.,Onn,S.P.,Grace,A.A.,Ouimet,C.C.,White,F.G.,Hyman,S.E.,Surmeier,D.G.,Girault,J.,Nestler,E.J.andGreengard,P.(1998)DARPP-32:regulatoroftheefficacyofdopaminergicneurotransmission.Science281:838-842.
LindskogM,SvenningssonP,PozziL,KimY,FienbergAA,BibbJA,FredholmBB,NairnAC,GreengardP,FisoneG(2002)InvolvementofDARPP-32phosphorylationinthestimulantactionofcaffeine.Nature(London)418:774-778.
MaldveRE,ZhangTA,Ferrani-KileK,SchreiberSS,LippmannMJ,SnyderGL,FeinbergAA,LeslieSW,GonzalesRA,MorrisettRA(2002)DARPP-32andtheregulationoftheethanolsensitivityofNMDAreceptorsinthenucleusaccumbens.NatureNeurosci5:641-648.
SvenningssonP,TzavaraET,WitkinJM,FienbergAA,NomikosGG,GreengardP(2002)InvolvementofstriatalandextrastriatalDARPP-32inbiochemicalandbehavioraleffectsoffluoxetine(Prozac®).ProcNatlAcadSciUSA99:3182-3187.
