Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- PPP1R1B
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1025-34,RRID:AB_2492068
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr34ofratDARPP-32.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
DARPP-32isadopamine(DA)andcAMP-regulated~32kphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Bonito-Oliva,A.,Södersten,E.,Spigolon,G.,Hu,X.,Hellysaz,A.,Falconi,A.,Gomes,A.L.,Broberger,C.,Hansen,K.,&Fisone,G.(2016).DifferentialregulationofthephosphorylationofTrimethyl-lysine27histoneH3atserine28indistinctpopulationsofstriatalprojectionneurons.Neuropharmacology,107,89-99. PMID:26947946
Beaulieu,J.M.(2015).Invivoveritas,thenextfrontierforfunctionallyselectiveGPCRligands.Methods.Aug28.PMID:26320830
Bonito-Oliva,A.,DuPont,C.,Madjid,N.,Ögren,S.O.,&Fisone,G.(2015).Involvementofthestriatalmediumspinyneuronsofthedirectpathwayinthemotorstimulanteffectsofphencyclidine.Theinternationaljournalofneuropsychopharmacology/(CINP).PMID:26657176
Del’Guidice,T.,&Beaulieu,J.M.(2015).SelectivedisruptionofdopamineD2-receptors/beta-arrestin2signalingbymoodstABIlizers.JournalofReceptorsandSignalTransduction,1-9.Jun;35(3):224-32.PMID:26459714
ThomasDel’Guidice,CamilleLatapy,AntonioRampino,JivanKhlghatyan,MorganeLemasson,BarbaraGelao,TizianaQuarto,GiuseppeRizzo,AnnieBarbeau,ClaudeLamarre,AlessandroBertolino,GiuseppeBlasi,andJean-MartinBeaulieu(2015)FXR1PisaGSK3βsubstrateregulatingmoodandemotionprocessing.PNAS,Aug18;112(33):E4610-9.PMID:26240334
DanielaMarazziti,ChiaraDiPietro,SilviaMandillo,ELISAbettaGolini,RafaeleMatteoni,andGlaucoP.Tocchini-Valentini(2011)AbsenceoftheGPR37/PAELreceptorimpairsstriatalAktandERK2phosphorylation,FosBexpression,andconditionedplacepreferencetoamphetamineandcocaine.FASEBJ,25:2071–2081.PMID:21372109
RajaniMaiya,YanZhou,ErinH.Norris,MaryJeanneKreek,andSidneyStrickland(2009)Tissueplasminogenactivatormodulatesthecellularandbehavioralresponsetococaine.PNAS,106:1983–1988.PMID:19181855
MichelleNiculescu,ShaneA.Perrine,JonathanS.Miller,MichelleE.Ehrlich,andEllenM.Unterwald(2008)Trk:ANeuromodulatorofAge-SpecificBehavioralandNeuRochemicalResponsestoCocaineinMice.J.Neurosci.,Jan2008;28:1198–1207.PMID:18234897
Jean-MartinBeaulieu,TatyanaD.Sotnikova,RaulR.Gainetdinov,andMarcG.Caron(2006)ParadoxicalStriatalCellularSignalingResponsestoPsychostimulantsinHyperactiveMice.J.Biol.Chem.,Oct2006;281:32072–32080.PMID:16954211
