Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- PPP1R1B
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1025-75,RRID:AB_2492069
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr75ofratDARPP-32.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
DARPP-32isadopamine(DA)andcAMP-regulated~32kDaphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
MichelleNiculescu,ShaneA.Perrine,JonathanS.Miller,MichelleE.Ehrlich,andEllenM.Unterwald(2008)Trk:ANeuromodulatorofAge-SpecificBehavioralandNeuRochemicalResponsestoCocaineinMice.J.Neurosci.,Jan2008;28:1198–1207. PMID:18234897
