Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:250
- Species:
- Rabbit
- GeneName:
- PPP1R1B
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#374-DARPP,RRID:AB_2492067
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofratDARPP-32,conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnpreparedwiththeN-terminalpeptideusedasantigen.
BIOLOGicalSignificance:Collapse
DARPP-32isadopamine(DA)andcAMP-regulated~32kDaphosphoproteinthatisassociatedwithdopaminoceptiveneurons(Fienbergetal.,1998).TheproteininhibitsproteinphosphataseIwhenitisphosphorylatedonThr34.Incontrast,whenDARPP-32isphosphorylatedonThr75theproteinactsasaninhibitorofPKA(Bibbetal.,1999).PhosphorylationofDARPP-32isthoughttoplayacriticalroleintheregulationofdopaminergicneurotransmission.Inaddition,theactivityofDARPP-32isalsothoughttoplayimportantrolesintheactionsofalcohol,caffeineandProzac®(Maldveetal.,2002;Lindskogetal.,2002;Svenningssonetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Bonito-Oliva,A.,DuPont,C.,Madjid,N.,Ögren,S.O.,&Fisone,G.(2015).Involvementofthestriatalmediumspinyneuronsofthedirectpathwayinthemotorstimulanteffectsofphencyclidine.Theinternationaljournalofneuropsychopharmacology/(CINP).PMID:26657176
MichelleNiculescu,ShaneA.Perrine,JonathanS.Miller,MichelleE.Ehrlich,andEllenM.Unterwald(2008)Trk:ANeuromodulatorofAge-SpecificBehavioralandNeuRochemicalResponsestoCocaineinMice.J.Neurosci.,Jan2008;28:1198–1207.PMID:18234897
