Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Bovine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- DDC
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#417-DDC,RRID:AB_2492072
Antigen/Purification:Collapse
TheantigenisanSDSdenatured,recombinantbovinearomaticDOPAdecarboxylase(DDC)expressedinE.coliandpurifiedfrominclusionbodies.
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusinganAminOlink®Pluscolumnmatrixtowhichpurified,recombinantbovineDDCwascoupled.
BIOLOGicalSignificance:Collapse
DOPAdecarboxylase(aromaticL-aminoaciddecarboxylase,AADC;DDC)catalyzesthesecondreactioninthebiosynthesisofcatecholaminesandSEROtonin(WaymireandHaycock,2002;Berryetal.,1996;Haycocketal.,2003).Itisalsoinvolvedinthebiosynthesisoftraceamines.DDCantibodiescanthereforebeusedasMarkersfordopaminergic,noradrenergicandserotonergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
HaycockJW,BeckerL,AngL,FurukawaY,HornykiewiczO,KishSJ(2003)Markeddisparitybetweenage-relatedchangesindopamineandotherpresynapticdopaminergicmarkersinhumanstriatum.JNeuRochem87:574-585.PMID:14535941
