Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- SLC6A3
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p435-53,RRID:AB_2492078
Antigen/Purification:Collapse
Theantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr53ofratDAT.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).IthasbeenshownthatphosphorylationatThr53directlyaffectsdopamineinfluxandamphetamine-stimulatedsubstrateefflux,indicatingthattheThr53residueplaysamajorroleintransportactivity(Fosteretal.,2012).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Sase,A.,Aher,Y.,Saroja,S.R.,Ganesan,M.K.,Sase,S.,Holy,M.,Hoger,H.,Bakulev,V.,Ecker,G.,Langer,T.,Sitte,H.H.,Leban,J.,&Lubec,G.(2015).AheterocycliccompoundCE-103inhibitsdopaminereuptakeandmodulatesdopaminetransporteranddopamineD1-D3containingreceptorcomplexes.Neuropharmacology.Sept22pii:S0028-3908(15)300046-0.PMID:26407764
JamesD.Foster,Jae-WonYang,AmyE.Moritz,SathyavathiChallaSivaKanaka,MargaretA.Smith,MarionHoly,KyleWilebski,HaraldH.Sitte,andRoxanneA.Vaughan(2012)DopamineTransporterPhosphorylationSiteThreonine53RegulatesSubstrateReuptakeandAmphetamine-stimulatedEfflux.JournalofBiologicalChemistry287(35):29702-29712.PMID:22722938
