Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Human,Non-humanprimate
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Rabbit
- GeneName:
- SLC6A3
- MolecularReference:
- ~88kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#431-DATC,RRID:AB_2492076
Antigen/Purification:Collapse
TheantigenisapeptidefromtheintracellularC-terminusregionofhumandopaminetransporter(DAT),conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationusingaSulfOlink®columnmatrixtowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Sarkar,S.,Gough,B.,Raymick,J.,Beaudoin,M.A.,Ali,S.F.,Virmani,A.,&Binienda,Z.K.(2015).Histopathologicalandelectrophysiologicalindicesofrotenone-evokeddopaminergictoxicity:Neuroprotectiveeffectsofacetyl-l-carnitine.Neuroscienceletters,606,53-59.PMID:26321151
KishSJ,KalasinskyKS,DerkachP,SchmunkGA,GuttmanM,AngL,AdamsV,FurukawaY,HaycockJW(2001)StriataldopaminergicandSEROtonergicmarkersinhumanheroinusers.Neuropsychopharmacology24:561-567.PMID:11282256
