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Functional_antibody >
PhosphoSolutions/Dopamine Transporter Antibody, Extracellular Loop 2/434-DATEL2/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Rabbit
- GeneName:
- SLC6A3
- MolecularReference:
- ~88kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#434-DATEL2,RRID:AB_2492077
Antigen/Purification:Collapse
Theantigenisapeptidefromtheextracellularloop2(EL2)regionofhumandopaminetransporter(DAT),conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingaSulfOlink®columnmatrixtowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Thedopaminetransporter(DAT)isresponsIBLeforthereaccumulationofdopamineafterithasbeenreleased.DATantibodiesandantibodiesforotherMarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).LevelsofDATproteinexpressionarealteredbychronicdrugadmiNISTration(Wilsonetal.,1996).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
ZhuMY,KlimekV,HaycockJW,OrdwayGA(2000)Quantitationoftyrosinehydroxylaseproteininthelocuscoeruleusfrompostmortemhumanbrain.JNeurosciMeth99:37-44.PMID:10936640
