MouseMonoclonalAntibody
- Size:
- 100µl
- Formulation:
- Mouseascitesfluid
- Specificity:
- Xenopus,Drosophila,Zebrafish,Mostmammals,Someinvertebrates
- Applications:
- WB1:5,000IF1:500IHC1:500
- Species:
- Mouse
- MolecularReference:
- ~42kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#125-ACT,RRID:AB_2492035
Antigen/Purification:Collapse
Theantigenisachickengizzardactin.
Theantibodyisunpurifiedascitesfluid.
BIOLOGicalSignificance:Collapse
Actinisthemostabundantproteinfoundinvirtuallyalleukaryoticcells.Itisalsooneofthemosthighly-conservedproteins,differingbynomorethan20%inspeciesasdiverseasalgaeandhumans,makingitanexcellentchoiceforuseasaloADIngcontrol.ItisthemonomericsubunitofMICROFILaments,oneofthethreemajorcomponentsoftheCytoskeleton,andofthinfilaments,whicharepartofthecontractileapparatusinmusclecells.Thus,actinparticipatesinmanyimportantcellularfunctions,includingmusclecontraction,cellmotility,celldivisionandcytokinesis,vesicleandorganellemovement,cellsignaling,andtheestablishmentandmaintenanceofcelljunctionsandcellshape.
Storage
Liquidcontaining0.01%sodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year. Avoidfreeze/thawcycles.
ProductSpecificReferences
Choi,Y.S.,Horning,P.,Aten,S.,Karelina,K.,Alzate-Correa,D.,Arthur,J.S.C.,Hoyt,K.R.andObrietan,K.,2017.Mitogen-andStress-ActivatedProteinKinase1RegulatesStatusEpilepticus-EvokedCellDeathintheHippocampus.ASNneuro,9(5),p.1759091417726607.PMID:28870089
Barcus,C.E.,O’Leary,K.A.,Brockman,J.L.,Rugowski,D.E.,Liu,Y.,Garcia,N.,Yu,M.,Keely,P.J.,Eliceiri,K.W.andSchuler,L.A.,2017.Elevatedcollagen-Iaugmentstumorprogressivesignals,intravasationandmetastasisofprolactin-inducedestrogenreceptoralphapositivemammarytumorcells.BreastCancerResearch,19(1),p.9.PMID:28103936
Ramena,G.,Yin,Y.,Yu,Y.,Walia,V.,&Elble,R.C.(2016).CLCA2interactorEVA1isrequiredformammaryepithelialcelldifferentiation.PloSone,11(3),e0147489.PMID:26930581
Goodfellow,M.J.,ABDulla,K.A.,&Lindquist,D.H.(2016).NeonatalethanolexposureimpairstracefearconditioningandaltersNMDAreceptorsubunitexpressioninadultmaleandfemalerats.Alcoholism:ClinicalandExperimentalResearch,40(2),309-318.PMID:26842250
Barcus,C.E.,Holt,E.C.,Keely,P.J.,Eliceiri,K.W.,&Schuler,L.A.(2015).DenseCollagen-IMatricesEnhancePro-TumOrigenicEstrogen-ProlactinCrosstalkinMCF-7andT47DBreastCancerCells.PloSone,10(1)e0116891.PMID:25607819
