Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgGfraction
- Specificity:
- Mostmammals
- Applications:
- WB1:2,000IF1:250IHC1:1,000
- Species:
- Mouse
- MolecularReference:
- ~66kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#100-AIN,RRID:AB_2492032
Antigen/Purification:Collapse
Theantigenisarecombinantratalpha-internexinexpressedinandpurifiedfromE.coli.
TheantibodyistotalIgGfraction.
BIOLOGicalSignificance:Collapse
Alpha-internexinisaClassIVintermediatefilamentoriginallydiscoveredasitco-purifieswithotherneurofilamentsubunits(1).Alpha-internexinisrelatedtobutdistinctfromthebetterknownneurofilamenttripletproteins,NF-L,NF-MandNF-H,havingsimilarproteinsequencemotifsandasimilarintronorganization.Itisexpressedonlyinneuronsandinlargeamountsearlyinneuronaldevelopment,butisdown-regulatedinmanyneuronsasdevelopmentproceeds.Manyclassesofmatureneuronscontainalpha-internexininadditiontoNF-L,NF-MandNF-H.Insomematureneuronsalphainternexinistheonlyneurofilamentsubunitexpressed.Antibodiestoalpha-internexinarethereforeuniqueprobestostudyandclassifyneuronaltypesandfollowtheirprocessesinsectionsandintissueculture.Inaddition,recentstudiesshowamarkedup-regulationofalpha-internexinduringneuronalregeneration(2).Theuseofantibodiestothisproteininthestudyofbraintumorshasnotbeenexaminedtodate,butislikelytobeofinterest.RecentlyCairnsetal.usedthisantibodytoshowthatalphainternexinisanabundantcomponentoftheinclusionsofneurofilamentinclusionbodydisease(NFID),aserioushumanneurodegenerativedisorder(3,4).Theantibodywasalsousedtoconfirmthepresenceofcirculatingauto-antibodiestoalpha-internexinintheseraofsomepatientswithendocrineautoimmunity,aswellasinsomenormalindividuals(5).
Storage
Liquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Pachter,JandLiem,RKH.Alpha-Internexin,a66-kDintermediatefilament-bindingproteinfrommammaliancentralnervoustissues.JCellBiol101:1316-22(1985).
McGrawetal.Axonallytransportedperipheralsignalsregulatealpha-internexinexpressioninregeneratingmotoneurons.JNeurosci22:4955-63(2002).
CairnsNJetal.alpha-Internexinaggregatesareabundantinneuronalintermediatefilamentinclusiondisease(NIFID)butrareinotherneurodegenerativediseases.ActaNeuropathol(Berl).May28[Epubaheadofprint](2004).
CairnsNJetal.alpha-internexinispresentinthepathologicalinclusionsofneuronalintermediatefilamentinclusiondisease.AmJPathol.164:2153-61(2004).
RajasaluT,TeesaluK,JanmeyPA,UiboR.Demonstrationofnaturalautoantibodiesagainsttheneurofilamentproteinalpha-internexininseraofpatientswithendocrineautoimmunityandhealthyindividuals.ImmunolLett.94:153-60(2004).
