Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGpurified
- Specificity:
- Bovine,Human,Mouse,Rat
- Applications:
- WB1:2000IF1:1000IHC1:1000
- Species:
- Mouse
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#309-cFOS,RRID:AB_2632380
Antigen/Purification:Collapse
Theantigenisrecombinantfulllengthhumanc-FOSexpressedinandpurifiedfromE.Coli.
TheantibodyisproteinGpurifiedfromculturesupernatant.
BIOLOGicalSignificance:Collapse
c-FOSisamemberoftheFOStranscriptionfactorfamilywhichformsdimerswithc-JUNtoproducetheActivatorProtein1(AP-1)complexwhichplaysakeyroleincriticalcellularprocessessuchascellproliferation,differentiationandapoptosis(Chiuetal.,1988). c-FOSexpressionhasbeendemonstratedtobeausefulMarkerofneuronalactivationasitisrapidlyinducedfollowingvariousstimuli(Hoffmanetal.,1993). Additionally,c-FOShasbeenshowntobeoverexpressedinavarietyofmalignanttumortypes(Milde-Langosch2005).
Storage
100µlinPBS+5mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Chiu,R.,Boyle,W.J.,Meek,J.,Smeal,T.,Hunter,T.,&Karin,M.(1988).Thec-fosproteininteractswithc-junAP-1tostimulatetranscriptionofAP-1responsivegenes.Cell,54(4),541-552.
Hoffman,G.E.,Smith,M.S.,&Verbalis,J.G.(1993).c-Fosandrelatedimmediateearlygeneproductsasmarkersofactivityinneuroendocrinesystems.Frontiersinneuroendocrinology,14(3),173-213.
Milde-Langosch,K.(2005).TheFosfamilyoftranscriptionfactorsandtheirroleintumourigenesis.Europeanjournalofcancer,41(16),2449-2461.
