Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Non-humanprimate,Sheep,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~66kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p142-477,RRID:AB_2492057
Antigen/Purification:Collapse
Theantigenisasyntheticphospho-peptidesurroundingthephosphoSer477ofhumanChe-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Che-1,alsoknownasAATF(apoptosis-antagonizingtranscriptionfactor),isaRNApolymeraseII-bindingproteininvolvedinregulatingthetranscriptionfactorE2Fandpromotingcellcycleprogression(Burgdorfetal.,2004).IthasbeensuggestedthatChe-1mayactasaneuroprotectivefactoragainstAbeta-inducedapoptosisbysuppressingtheproductionofreactiveoxidativespecies(Xieetal.,2004).ThecheckpointkinaseChk2hasbeenshowntophosphorylateChe-1atSer477contributingtothemaintenanceoftheG2/McheckpointinducedbyDNAdamage(Brunoetal.,2006).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BurgdorfS,LeisterP,ScheidtmannKH.(2004)TSG101interactswithapoptosis-antagonizingtranscriptionfactorandenhancesandrogenreceptor-mediatedtranscriptionbypromotingitsmonoubiquitination.JBiolChem.279(17):17524-34
XieJ,GuoQ(2004)AATFprotectsneuralcellsagainstoxidativedamageinducedbyamyloidbeta-peptide.NeurobiolDis.16(1):150-7
BrunoT,DeNicolaF,IezziS,LecisD,D’AngeloC,DiPadovaM,CorbiN,DimizianiL,ZanniniL,JekimovsC,ScarsellaM,PorrelloA,ChersiA,CrescenziM,LeonettiC,KhannaKK,SodduS,FloridiA,PassanantiC,DeliaD,FanciulliM.(2006)Che-1phosphorylationbyATM/ATRandChk2kinasesactivatesp53transcriptionandtheG2/Mcheckpoint.CancerCell.10(6):473-86
