RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1012-326,RRID:AB_2651147
Antigen/Purification:Collapse
Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser326ofhumanCtIP.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
CtIP,C-terminalbindingprotein-interactingprotein,isaDNAendonucleaseactivatedbydoublestrandedbreaks(DSBs). DSBrepairscanbeperformedbyeitheroneoftwomechanisms;non-homologousendjoining(NHEJ)orhomologousrecombination(HR). NHEJisthepredominantDSBrepairpathwaythroughouttheentirecellcycle,mostimportantlyintheG1phase(Rothkammetal,2003);whileHRisimportantforrepairingDSBsinSandG2phases(Beucheretal,2009).CtIPcontrolsDSBresection;aneventthatonlyoccursinHRduringG2-phase.PhosphorylationofThr847dictatestheresectionefficiency(Huertasetal,2008).FurThermore,ithasbeenfoundthatDSBsundergoresectionandrepairinG1-phasecellsviaaprocessrequiringPlk3phosphorylationofCtIPatSer327andThr847(Bartonetal,2014). SeveraladditionalphosphorylationsiteswithinCtIPhavebeenidentified,buttheirsignificanceintherepairofDNAhaveyettobedetermined.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
RothkammK,KrugerI,ThompsonLH,LobrichM(2003)PathwaysofDNAdouble-strandbreakrepairduringthemammaliancellcycle.MolCellBiol.,23,5706-5715.
BeucherA,BirrauxJ,tchouandongL,BaronO,ShibataA,ConradS,GoodarziAA,KremplerA,JeggoPA,LobrichM(2009)ATMandArtemispromotehomologousrecombinationofrADIation-inductedDNAdouble-strandbreaksinG2.EMBOJ.,28,3413-3427.
HuertasP,JacksonSP(2008)HumanCtIPMediatesCellCycleControlofDNAEndResectionandDoubleStrandBreakRepair.J.Biol.Chem,284:9558-9565.
OliviaBarton,SteffenCNaumann,RonjaDiemer-Biehs,JuliaKünzel,MonikaSteinlage,SandroConrad,NodarMakharashili,JiadongWang,LinFent,BernardS.Lopez,TanyaT.Paull,JunjieChen,PennyAJeggo,andMarkusLöbrich(2014)Polo-likeKinase3regulatesCtIPduringDNAdouble-strandbreakrepairinG1.JCB, Nov7;206(7):877-894.
