Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Bovine,Non-humanprimate,Sheep,Goat
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~150kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p171-556,RRID:AB_2492092
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer556ofhumanFANCI.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Fanconianemia,FA,isararedisorderwherecellscannotprevent,repair,ortolerateDNAdamage,leADIngtocancer,progressivebonemarrowfailureanddevelopmentalabnormalities(Ishiaietal,2008).16geneshavebeenimplicatedinFA,andtheirproductsconstituteacommonFApathway(WaldenandDeans2014).FANCI(FanconianemiacomplementationgroupI),isoneoftwosubstratesformonoubiquitinationbytheFANCL-containingcorecomplex,andiscrucialforDNArepairviaFAPandintrastrandcross-links(WaldenandDeans,2014).PhosphorylationatSer556hasbeenshowntoplayakeyroleinFANCIphysicallyassociatingwithFANCD2,stABIlizingtheID2complex,andsupportingitsDNAbindingandsubsequentD2-ubiquitination(WaldenandDeans,2014).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Cheung,R.S.,Castella,M.,Abeyta,A.,Gafken,P.R.,Tucker,N.andTaniguchi,T.,2017.Ubiquitination-LinkedPhosphorylationoftheFANCIS/TQClusterContributestoActivationoftheFanconiAnemiaI/D2Complex.CellReports,19(12),pp.2432-2440.PMID:28636932
