Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Sheep
- Applications:
- WB1:1,000IHC1:100
- Species:
- Rabbit
- MolecularReference:
- ~140kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#322-COLT,RRID:AB_2492061
Antigen/Purification:Collapse
TheantigenisasyntheticpeptidefromtheC-terminalregionofhumancollagenIα1telopeptidesequence.
Theantibodyisaffinitypurified.
BIOLOGicalSignificance:Collapse
Collagenisanextracellularmatrixproteinthatservesasascaffolddefiningtheshapeandmechanicalpropertiesofmanytissuesandorgansincludingskin,tendon,arterywalls,fibrocartilage,boneandteeth.Type1collagenisthemostabundantproteininmammals.CollagensaresynthesizedwithN-terminalandC-terminalpropeptidesthatarecleavedduringmaturationandsecretion.Aftercleavageofthepropeptides,themostN-terminalandC-terminalremainingsequencesareknownastelopeptides.Mutationsinthecollagen1,alpha1gene(COL1A1)areknowntocauseosteogenesisimperfecta(akabrittlebonedisease)(Byers1989).FurThermore,mutationsfoundinthefirst90residuesofthehelicalregionofalpha1collagenhavebeenimplicatedinthepreventionordelayedremovaloftheprocollagenN-propeptideleADIngtoacombinedosteogenesisimperfectaandEhlers-Danlossyndrome(EDS)phenotype(Cabraletal.,2005).
Storage
LiquidinPBS.Containsnopreservative.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cuptosixmonthsafterdateofreceipt.Avoidfreeze/thawcycles.
ProductSpecificReferences
ReeseC,LeeR,BonnerM,PerryB,HeywoodJ,SilverRM,TourkinaE,ViscontiRP,HoffmanS.(2014)Fibrocytesinthefibroticlung:alteredphenotypedetectedbyflowcytometry.FrontPharmacol.2014Jun16;5:141.PMID:24999331
Bains,SN,TourkinaE,AtkinsonC,JosephK,TholanikunnelB,ChuHW,RiemerEC,MartinR,HoffmanS(2012)Lossofcaveolin-1frombronchialepithelialcellsandmonocytesinhumansubjectswithasthma.Allergy.2012Dec;67(12):1601-4. PMID:23004679
WesselsA,vandenHoffMJ,AdamoRF,PhelpsAL,LockhartMM,SaulsK,BriggsLE,NorrisRA,vanWijkB,Perez-PomaresJM,DettmanRW,BurchJB.(2012)Epicardiallyderivedfibroblastspreferentiallycontributetotheparietalleafletsoftheatrioventricularvalvesinthemurineheart.DevBiol.2012Jun15;366(2):111-24.PMID:22546693
