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Functional_antibody >
PhosphoSolutions/GABA Transporter (GAT) 1 Antibody/880-GAT1/100 µl
Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000IHC1:100-1:200
- Species:
- Rabbit
- GeneName:
- SLC6A1
- MolecularReference:
- ~67kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#880-GAT1,RRID:AB_2492119
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratGAT-1.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviachromatographywithacolumnmadewiththepeptideusedasantigen.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAplasmamembranetransporters(GATs)influencesynapticneurotransmissionbyhighaffinityuptakeandreleaseofGABA.Todate,fourdistinctGABAtransportershavebeenidentified:GAT-1,GAT-2,GAT-3,andBGT-1.GAT-1,themostabundantofthetransporters,isfoundpredominantlyinneurons,butalsoinsomespecializedglia(Minellietal.,1995).GAT-1isthoughttoplayakeyroleinepileptogenesis(Zhaoetal.2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReference
Johnson,J.,Chen,T.K.,Rickman,D.W.,Evans,C.,&Brecha,N.C.(1996).Multipleγ-aminobutyricacidplasmamembranetransporters(GAT-1,GAT-2,GAT-3)intheratretina.TheJournalofcomparativeneurology,375(2),212.PMID:8915826
