Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRA1
- MolecularReference:
- ~51kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#811-GA1C,RRID:AB_2492099
Antigen/Purification:Collapse
Theantigenisafusionproteinfromthecytoplasmicloopoftheα1-subunitofratGABAAreceptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsub-stanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Wang,P.,Eshaq,R.S.,Meshul,C.K.,Moore,C.,Hood,R.L.,&Leidenheimer,N.J.(2015).Neuronalgamma-aminobutyricacid(GABA)typeAreceptorsundergocognateligandchaperoningintheendoplasmicreticulumbyendogenousGABA.FrontiersinCellularNeuroscience,9,188.PMID:26041994
Herman,M.A.,&Roberto,M.(2014).Cell‐type‐specifictonicGABAsignalingintheratcentralamygdalaisselectivelyalteredbyacuteandchronicethanol.Addictionbiology.Aug.29.PMID:25170988
MridulaRewal,RachelJurd,T.MichaelGill,Dao-YaoHe,DoritRon,andPatriciaH.Janak(2009)alpha-4-ContainingGABAAReceptorsintheNucleusAccumbensMediateModerateIntakeofAlcoholJ.Neurosci.,29:543-549.PMID:19144854
