Rabbitpolyclonalantibody
*Thisantibodyisareplacementforouroriginalcatalog#822-GA2C.Itwas
producedbythesamemethods,usingthesamefusionproteinantigenin
newanimals.
- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRA2
- MolecularReference:
- ~51kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#822-GA2CL,RRID:AB_2492101
Antigen/Purification:Collapse
Theantigenisafusionproteinfromthecytoplasmicloopoftheα2-subunitofratGABAAreceptor.
Theantibodyis preparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Engin,E.,Zarnowska,E.D.,Benke,D.,Tsvetkov,E.,Sigal,M.,Keist,R.,Bolshakov,V.Y.,Pearce,R.A.,&Rudolph,U.(2015).TonicInhibitoryControlofDentateGyrusGranuleCellsbyα5-ContainingGABAAReceptorsReducesMemoryInterference.TheJournalofNeuroscience,35(40),13698-13712.PMID:26446222
