Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRA5
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#846-GA5C,RRID:AB_2492105
Antigen/Purification:Collapse
Theantigenisafusionproteinfromthecytoplasmicloopoftheα5-subunitofratGABAAreceptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAA receptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Engin,E.,Zarnowska,E.D.,Benke,D.,Tsvetkov,E.,Sigal,M.,Keist,R.,Bolshakov,V.Y.,Pearce,R.A.,&Rudolph,U.(2015).TonicInhibitoryControlofDentateGyrusGranuleCellsbyα5-ContainingGABAAReceptorsReducesMemoryInterference.TheJournalofNeuroscience,35(40),13698-13712.PMID:26446222
Centanni,S.W.,Teppen,T.,Risher,M.L.,Fleming,R.L.,Moss,J.L.,Acheson,S.K.,Mulholland,P.J.,Pandey,S.C.,Chandler,L.J.,&Swartzwelder,H.S.(2014).AdolescentalcoholexposurealtersGABAAreceptorsubunitexpressioninadulthippocampus.Alcoholism:ClinicalandExperimentalResearch,38(11),2800-2808.PMID:25421517
Zurek,A.A.,Yu,J.,Wang,D.S.,Haffey,S.C.,Bridgwater,E.M.,Penna,A.,Lecker,I.,Lei,G.,Chang,T.,Salter,E.W.andOrser,B.A.(2014).Sustainedincreaseinα5GABAAreceptorfunctionimpairsmemoryafteranesthesia.TheJournalofclinicalinvestigation,124(12),p.5437. PMID:25365226
