Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Rat,Human,Zenopus
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- CAMK2A/B
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1005-286,RRID:AB_2492051
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr286foundinratbrainCaMKinaseII.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Ca2+/calmodulin-dependentproteinkinaseII(CaMKinaseII)isamulti-functionalcalciumandcalmodulin-dependentproteinkinasethatmediatescellularresponsestoawidevarietyofintercellularsignals(Kennedy,1998;SchulmanandHanson,1993).CaMKinaseIIhasbeenshowntoregulatediversecellularfunctionsincludingsynapticplasticity,neurotransmittersynthesisandrelease,geneexpression,ionchannelfunction,carbohydratemetabolism,cytoskeletalfunction,andCa2+-homeostasis(Gleasonetal.,2003;Soderling,2000;HudmonandSchulman,2002).PhosphorylationofThr286onthekinaseproducesanautonomouslyactiveformofCaMKinaseII(Mengetal.,2003;Picciottoetal.,1993).AutophosphorylationofThr305inhibitstheactivityCaMKinaseII.PhosphorylationatthissiteappearstoreducetheassociationofCaMKinaseIIwiththePSDandreduceLTPandlearning(Elgersmaetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Murakoshi,H.,Shin,M.E.,Parra-Bueno,P.,Szatmari,E.M.,Shibata,A.C.andYasuda,R.,2017.KineticsofEndogenousCaMKIIRequiredforSynapticPlasticityRevealedbyOptogeneticKinaseInhibitor.Neuron.Apr5;94(1):37-47.e5.PMID:28318784
Huang,L.,Hayes,S.andYang,G.,2016.Long-lastingbehavioraleffectsinneonatalmicewithmultipleexposuresto…-xylazineanesthesia.NeurotoxicologyandTeratology.PMID:27622724
Barcomb,K.,Buard,I.,Coultrap,S.J.,Kulbe,J.R.,O’Leary,H.,Benke,T.A.,&Bayer,K.U.(2014).AutonomousCaMKIIrequiresfurtherstimulationbyCa2+/calmodulinforenhancingsynapticstrength.TheFASEBJournal,28(8),3810-3819.PMID:24843070
Bernard,P.B.,Castano,A.M.,Bayer,K.U.,&Benke,T.A.(2014).Necessary,butnotsufficient:insightsintothemechanismsofmGluRmediatedlong-termdepressionfromaratmodelofearlylifeseizures.Neuropharmacology,84,1-12.PMID:24780380
StevenJ.Coultrap,K.UlrichBayer(2014)NitricoxideinducesCa2+-independentactivityoftheCa2+/calmodulin-dependentproteinkinaseII(CaMKII).J.BiolChem.289(28):19458-65.PMID:24855644
Magupalli,V.G.,Mochida,S.,Yan,J.,Jiang,X.,Westenbroek,R.E.,Nairn,A.C.,Scheuer,T.,&Catterall,W.A.(2013).Ca2+-independentactivationofCa2+/calmodulin-dependentproteinkinaseIIboundtotheC-terminaldomainofCaV2.1calciumchannels.JournalofBiologicalChemistry,288(7),4637-4648.PMID: 23255606
W.WadeKothmann,E.BradyTrexler,ChristopherM.Whitaker,WeiLi,StephenC.Massey,andJohnO’Brien(2012)NonsynapticNMDAReceptorsMediateActivity-DependentPlasticityofGapJunctionalCouplingintheAllAmacrineCellNetwork.J.Neurosci.32(3):6747-6759.PMID:22593045
RebekahS.Vest,HeatherO’Leary,StevenJ.Coultrap,MarkS.Kindy,andK.UlrichBayer(2010)EffectivePost-insultNeuroprotectionbyaNovelCa2+/Calmodulin-dependentProteinKinaseII(CaMKII)InhibitorJ.Biol.Chem.,285:20675–20682.PMID:20424167
StevenJ.Coultrap,IsabelleBuard,JaquelineR.Kulbe,MarkL.Dell’Acqua,andK.UlrichBayer(2010)CaMKIIAutonomyIsSubstrate-dependentandFurtherStimulatedbyCa2+/CalmodulinJ.Biol.Chem.,285:17930–17937.PMID:20353941
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNotAmino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
XiuSun,MichaelMilovanovic,YunZhao,andMarinaE.Wolf(2008)AcuteandChronicDopamineReceptorStimulationModulatesAMPAReceptorTraffickinginNucleusAccumbensNeuronsCoculturedwithPrefrontalCortexNeurons.J.Neurosci.,28:4216–4230.PMID:18417701
MatthewTownsend,TapanMehta,andDennisJ.Selkoe(2007)SolubleA-betaInhibitsSpecificSignalTransductionCascadesCommontotheInsulinReceptorPathway.J.Biol.Chem.,282:33305–33312.PMID:17855343
RebekahS.Vest,KurtisD.Davies,HeatherO’Leary,J.DavidPort,andK.UlrichBayer(2007)DualMechanismofaNaturalCaMKIIInhibitor.Mol.Biol.Cell,18:5024–5033.PMID:17942605
