Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Zebrafish,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- CAMK2A/B
- MolecularReference:
- ~50kDaand~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1005-306,RRID:AB_2560940
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr306foundinratbrainCaMKinaseII.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Ca2+/Calmodulin-DependentProteinKinaseII(CaMKinaseII)isamultifunctionalcalciumandcalmodulin-dependentproteinkinasethatmediatescellularresponsestoawidevarietyofintercellularsignals(Kennedy,1998;SchulmanandHanson,1993).CaMKinaseIIhasbeenshowntoregulatediversecellularfunctionsincludingsynapticplasticity,neurotransmittersynthesisandrelease,geneexpression,ionchannelfunction,carbohydratemetabolism,cytoskeletalfunction,andCa2+-homeostasis(Gleasonetal.,2003;Soderling,2000;HudmonandSchulman,2002).PhosphorylationofThr286onthekinaseproducesanautonomouslyactiveformofCaMKinaseII(Mengetal.,2003;Picciottoetal.,1993).CaMKIIαautophosphorylationatThr286andThr305/Thr306hasrecentlybeenshowntoregulatekinaseactivityandmodulatesubcellulartargetingandiscriticalfornormalsynapticplasticityandlearningandmemory(Baucumetal.,2015).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BaucumAJ,ShonesyBC,RoseKL,ColbranRJ(2015)QuantitativeProteomicsAnalysisofCaMKIIPhosphorylationandtheCaMKIIInteractomeintheMouseForebrain.ACSChemNeurosci.2015Feb24.
ElgersmaY,FedorovNB,IkonenS,ChoiES,ElgersmaM,CarvalhoOM,GieseKP,SilvaAJ(2002)InhibitoryautophosphorylationofCaMKIIcontrolsPSDassociation,plasticity,andlearning.Neuron36:493-505.
GleasonMR,HigashijimaS,DallmanJ,LiuK,MandelG,FetchoJR(2003)TranslocationofCaMkinaseIItosynapticsitesinvivo.NatureNeurosci6:217-218.
HudmonA,SchulmanH(2002)NeuronalCa2+/calmodulin-dependentproteinkinaseII:Theroleofstructureandautoregulationincellularfunction.AnnuRevBiochem71:473-510.
KennedyMB(1998)Signaltransductionmoleculesattheglutamatergicpostsynapticmembrane.BrainResRev26:243-257.
MengFJ,GuoJ,ZhangQG,SongB,ZhangGY(2003)Autophosphorylatedcalcium/calmodulin-dependentproteinkinaseIIa(CaMKIIa)reversIBLytargetstoandphosphorylatesN-methyl-D-aspartatereceptorsubunit2B(NR2B)incerebralischemiaandreperfusioninhippocampusofrats.BrainRes967:161-169.
PicciottoMR,CzernikAJ,NairnAC(1993)Calcium/calmodulin-dependentproteinkinaseI.CDNAcloningandidentificationofautophosphorylationsite.JBiolChem268:26512-26521.
SchulmanH,HansonPI(1993)MultifunctionalCa2+/calmodulin-dependentproteinkinase.NeuRochemRes18:65-77.
SoderlingTR(2000)CaM-kinases:modulatorsofsynapticplasticity.CurrOpinNeurobiol10:375-380.
