Rabbitpolyclonalantibody
PooledSerum- Size:
- 150µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Chicken,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GRIA1
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1160-831,RRID:AB_2492127
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser831ofGluR1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR)whilethoseactivatedbyα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownasAMPAreceptors(AMPAR).TheAMPARarecomprisedoffourdistinctglutamatereceptorsubunitsdesignated(GluR1-4)andtheyplaykeyrolesinvirtuallyallexcitatoryneurotransmissioninthebrain(Keinänenetal.,1990;HollmannandHeinemann,1994).TheGluR1subunitiswidelyexpressedthroughoutthenervoussystem.GluR1ispotentiatedbyphosphorylationatSer831whichhasbeenshowntobemediatedbyeitherPKCorCaMkinaseII(McGlade-McCullohetal.,1993;Mammenetal.,1999;Rocheetal.,1996).Inaddition,phosphorylationofthissitehasbeenlinkedtosynapticplasticityaswellaslearningandmemory(SoderlingandDerkach,2000).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Lameth,J.,Gervais,A.,Colin,C.,Lévêque,P.,Jay,T.M.,Edeline,J.M.andMallat,M.,2017.AcuteNeuroinflammationPromotesCellResponsesto1800MHzGSMElectromagneticFieldsintheRatCerebralCortex.NeurotoxicityResearch,pp.1-16.PMID:28578480
O’Leary,H.,Bernard,P.B.,Castano,A.M.,&Benke,T.A.(2016).EnhancedlongtermpotentiationanddecreasedAMPAreceptordesensitizationintheacuteperiodfollowingasinglekainateinducedearlylifeseizure.Neurobiologyofdisease,87,134-144.PMID:26706598
Xue,B.,Chen,E.C.,He,N.,Jin,D.Z.,Mao,L.M.,&Wang,J.Q.(2016).IntegratedregulationofAMPAglutamatereceptorphosphorylationinthestriatumbydopamineandacetylcholine.Neuropharmacology.PMID:27060412
Mao,L.M.,Xue,B.,Jin,D.Z.,&Wang,J.Q.(2015).DynamicincreasesinAMPAreceptorphosphorylationintherathippocampusinresponsetoamphetamine.Journalofneurochemistry.Jun;133(6):795-805.PMID:25689263
Pasek,J.G.,Wang,X.,&Colbran,R.J.(2015).DifferentialCaMKIIregulationbyvoltage-gatedcalciumchannelsinthestriatum.MolecularandCellularNeuroscience,68,234-243. PMID:26255006
Mao,L.M.,Hastings,J.M.,Fibuch,E.E.,&Wang,J.Q.(2014).PropofolselectivelyaltersGluA1AMPAreceptorphosphorylationinthehippocampusbutnotprefrontalcortexinyoungandagedmice.Europeanjournalofpharmacology,738,237-244. PMID:24907515
Xue,B.,Edwards,M.C.,Mao,L.M.,Guo,M.L.,Jin,D.Z.,Fibuch,E.E.,&Wang,J.Q.(2014).RapidandsustainedGluA1S845phosphorylationinsynapticandextrasynapticlocationsintheratforebrainfollowingamphetamineadmiNISTration.Neurochemistryinternational,64,48-54. PMID:24231469
Barcomb,K.,Coultrap,S.J.,&Bayer,K.U.(2013).EnzymaticactivityofCaMKIIisnotrequiredforitsinteractionwiththeglutamatereceptorsubunitGluN2B.Molecularpharmacology,84(6),834-843.PMID:24056996
YuxiaJiao,NidhiJalan-Sakrikar,A.J.Robison,AnthonyJ.Baucum,II,MarthaA.Bass,andRogerJ.Colbran(2011)CharacterizationofaCentralCa2+/Calmodulin-dependentProteinKinaseIIα/βBindingDomaininDensinThatSelectivelyModulatesGlutamateReceptorSubunitPhosphorylation.J.Biol.Chem.286:24806–24818. PMID:21610080
StevenJ.Coultrap,IsabelleBuard,JaquelineR.Kulbe,MarkL.Dell’Acqua,andK.UlrichBayer(2010)CaMKIIAutonomyIsSubstrate-dependentandFurtherStimulatedbyCa2+/Calmodulin.J.Biol.Chem.,285:17930–1793. PMID:20353941
RobertM.Sears,Rong-JianLiu,NandakumarS.Narayanan,RuthSharf,MarkF.Yeckel,MarkLaubach,GeorgeK.Aghajanian,andRalphJ.DiLeone(2010)RegulationofNucleusAccumbensActivitybytheHypothalamicNeuropeptideMelanin-ConcentratingHormoneJ.Neurosci.,30:8263–8273.PMID:20554878
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNotα-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
