Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Chicken,Non-humanprimate,Zebrafish
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- GRIA2
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1170-880,RRID:AB_2492131
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer880ofratGluR2.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR)whilethoseactivatedbyα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownasAMPAreceptors(AMPAR).TheAMPARarecomprisedoffourdistinctglutamatereceptorsubunitsdesignated(GluR1-4)andtheyplaykeyrolesinvirtuallyallexcitatoryneurotransmissioninthebrain(Keinänenetal.,1990;HollmannandHeinemann,1994).ThenumberofGluR2subunitsintheAMPAreceptorcomplexaffectstheCa2+permeABIlity,rectificationandsingle-channelconductanceofAMPAreceptors.Ser880hasbeenidentifiedasthePKCphosphorylationsitewithintheC-terminalregionofGluR2andhasbeenshowntodifferentiallyregulatetheinteractionofthePDZdomain-containingproteinsGRIP1andPICK1(Matsudaetal.,1999)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended. Storeat–20°C;stableforatleastoneyear.
ProductSpecificReferences
Lameth,J.,Gervais,A.,Colin,C.,Lévêque,P.,Jay,T.M.,Edeline,J.M.andMallat,M.,2017.AcuteNeuroinflammationPromotesCellResponsesto1800MHzGSMElectromagneticFieldsintheRatCerebralCortex.NeurotoxicityResearch,pp.1-16.PMID:28578480
Singer,B.F.,Bubula,N.,Przybycien-Szymanska,M.M.,Li,D.andVezina,P.,2016.Stimuliassociatedwiththepresenceorabsenceofamphetamineregulatecytoskeletalsignalingandbehavior.EuropeanNeuropsychopharmacology.PMID:27720500
O’Leary,H.,Bernard,P.B.,Castano,A.M.,&Benke,T.A.(2016).EnhancedlongtermpotentiationanddecreasedAMPAreceptordesensitizationintheacuteperiodfollowingasinglekainateinducedearlylifeseizure.Neurobiologyofdisease,87,134-144.PMID:26706598
Xue,B.,Edwards,M.C.,Mao,L.M.,Guo,M.L.,Jin,D.Z.,Fibuch,E.E.,&Wang,J.Q.(2014).RapidandsustainedGluA1S845phosphorylationinsynapticandextrasynapticlocationsintheratforebrainfollowingamphetamineadmiNISTration.NeuRochemistryinternational,64,48-54.PMID:24231469
