Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Chicken,Non-humanprimate,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GRIA2
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#900-GluR2,RRID:AB_2492129
Antigen/Purification:Collapse
Theantigenisa peptidecorrespondingtoaminoacidresiduesfromratGluR2,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationonacolumnmadewiththepeptideantigen.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR)whilethoseactivatedbyα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownasAMPAreceptors(AMPAR).TheAMPARarecomprisedoffourdistinctglutamatereceptorsubunitsdesignated(GluR1-4)andtheyplaykeyrolesinvirtuallyallexcitatoryneurotransmissioninthebrain(Keinänenetal.,1990;HollmannandHeinemann,1994).TheGluR2subunitisthoughttoplayakeyroleinformsofsynapticplasticitysuchasLTD(Chungetal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Lameth,J.,Gervais,A.,Colin,C.,Lévêque,P.,Jay,T.M.,Edeline,J.M.andMallat,M.,2017.AcuteNeuroinflammationPromotesCellResponsesto1800MHzGSMElectromagneticFieldsintheRatCerebralCortex.NeurotoxicityResearch,pp.1-16.PMID:28578480
