Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Chicken,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GRIA2/3
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#905-GluR2/3,RRID:AB_2492130
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratGluR2/3.
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnmadewiththeC-terminalpeptideusedasantigen.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR)whilethoseactivatedbyα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownasAMPAreceptors(AMPAR).TheAMPARarecomprisedoffourdistinctglutamatereceptorsubunitsdesignated(GluR1-4)andtheyplaykeyrolesinvirtuallyallexcitatoryneurotransmissioninthebrain(Keinänenetal.,1990;HollmannandHeinemann,1994).TheGluR2subunitiswidelyexpressedthroughoutthenervoussystemwhereitisthoughttoplaykeyrolesinsynapticplasticityandlearningandmemory(Dupratetal.,2003;Seidenmanetal.,2003;Chungetal.,2003;Yanetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
ChungHJ,SteinbergJP,HuganirRL,LindenDJ(2003)RequirementofAMPAreceptorGluR2phosphorylationforcerebellarlong-termdepression.Science300:1751-1755.
DupratF,DawM,LimW,CollingridgeG,IsaacJ(2003)GluR2protein-proteininteractionsandtheregulationofAMPAreceptorsduringsynapticplasticity.PhilosTransRSocLondBBiolSci358:715-720.
HollmannM,HeinemannS(1994)Clonedglutamatereceptors.AnnuRevNeurosci17:31-108.
KeinänenK,WisdenW,SommerB,WernerP,HerbA,VerdoornTA,SakmannB,SeeburgPH(1990)AfamilyofAMPA-selectiveglutamatereceptors.Science249:556-560.
SeidenmanKJ,SteinbergJP,HuganirR,MalinowR(2003)Glutamatereceptorsubunit2serine880phosphorylationmodulatessynaptictransmissionandmediatesplasticityinCA1pyramidalcells.JNeurosci23:9220-9228.
YanJ,ZhangYF,JiaZP,TavernaFA,McDonaldRJ,MullerRU,RoderJC(2002)Place-cellimpairmentinglutamatereceptor2mutantmice.JNeurosci22:NIL7-NIL11.
