![磷酸溶液/GRIP1相关蛋白1(GRAP1)抗体/642-GRAP/100µl](https://www.ebiomall.cn/images/phosphosolutions/642-GRASP-WB-CNC-e1455463858740.gif)
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~95kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#642-GRASP,RRID:AB_2492135
Antigen/Purification:Collapse
Theantigenisa peptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratGRASP1,conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfromrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnpreparedwiththeC-terminalpeptideusedasantigen.
BIOLOGicalSignificance:Collapse
PDZdomain-containingproteins,suchasPSD-95andGRIParethoughttoplaykeyrolesinglutamatereceptorplasticity.GRIP-associatedproteins(GRASPs)thatbindtodistinctPDZdomainswithinGRIPalsoplaykeyrolesinregulationofglutamatereceptorfunction.GRASP-1isaneuronalrasGEFassociatedwithGRIPandAMPAreceptorsinvivo(ScannevinandHuganir,2000).RecentworksuggeststhatGRASP-1mayregulateneuronalrassignalingandcontributetotheregulationofAMPAreceptordistributionbyNMDAreceptoractivity(Yeetal.,2000).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
ScannevinRH,HuganirRL(2000)Postsynapticorganizationandregulationofexcitatorysynapses.NatRevNeurosci1:133-141.YeB,LiaoDZ,ZhangXQ,ZhangPS,DongHL,HuganirRL(2000)GRASP-1:AneuronalRasGEFassociatedwiththeAMPAreceptor/GRIPcomplex.Neuron26:603-617.