Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Rabbit
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- TPH1
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1575-58,RRID:AB_2492273
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser58oftryptophanHydroxylase1(TPH1).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Tryptophanhydroxylase(TPH)catalyzesthe5-hydroxylationoftryptophan,whichisthefirststepinthebiosynthesisofindoleamines(SEROtoninandmelatonin)(Martinezetal.,2001).Inmammals,serotoninbiosynthesisoccurspredominantlyinneuronswhichoriginateintheRaphenucleiofthebrain,andmelatoninsynthesistakesplacewithinthepinealgland.AlthoughTPHcatalyzesthesamereactionwithintheRaphenucleiandthepinealgland,TPHactivityisrate-limitingforserotoninbutnotmelatoninbiosynthesis.Serotoninfunctionsmainlyasaneurotransmitter,whereasmelatoninistheprincipalhormonesecretedbythepinealgland.TheactivityofTPHisenhancedbyphosphorylationbycAMP-dependentproteinkinase(PKA)andCa2+/calmodulinkinaseII(CaMKII)(Jiangetal.,2000;Johansenetal.,1996)BothPKAandCaMKIIphosphorylateSer58whichlieswithintheregulatorydomainofTPH(Kuhnetal.,1997).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
JiangGC,YohrlingGJ,SchmittJD,VranaKE(2000)Identificationofsubstrateorientingandphosphorylationsiteswithintryptophanhydroxylaseusinghomology-basedmolecularmodeling.JMolBiol302:1005-1017.
JohansenPA,JenningsI,CottonRG,KuhnDM(1996)PhosphorylationandactivationoftryptophanhydroxylasebyexogenousproteinkinaseA.JNeuRochem66:817-823.
Kuhn,DM,Arthur,Jr,R,States,JC(1997)Phosphorylationandactivationofbraintryptophanhydroxylase:identificationofserine-58asasubstratesiteforproteinkinaseA.JNeurochem68:2220-2223.
MartinezA,KnappskogPM,HaavikJ(2001)Structuralapproachintohumantryptophanhydroxylaseanditsimplicationsfortheregulationofserotoninbiosynthesis.CurrMedChem8:1077-1091.
