Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Human
- Applications:
- WB1:1000IHC1:1000
- Species:
- Sheep
- GeneName:
- TPH1
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2015-TPH,RRID:AB_2492271
Antigen/Purification:Collapse
Theantigenisarecombinantrabbittryptophanhydroxylase,isolatedasinclusionbodiesfromE.coliandpurifiedbypreparativeSDS-PAGE.
Theantibodyispreparedfrompooledsheepserumbyaffinitypurificationusingacolumntowhichtherecombinantproteinwascoupled.
BIOLOGicalSignificance:Collapse
Tryptophanhydroxylase(TPH)catalyzesthefirststepinthebiosynthesisofSEROtoninandmelatonin(Martinezetal.,2001).Thus,expressionofTPHcanbeusedasanindicatorofthelocalizationofserotoninandmelatonininbrain.Inmammals,serotoninbiosynthesisoccurspredominantlyinneuronswhichoriginateintheRaphenucleiofthebrain,andmelatoninsynthesistakesplacewithinthepinealgland(Haycocketal.,2002).AlthoughTPHcatalyzesthesamereactionwithintheRaphenucleiandthepinealgland,TPHactivityisrate-limitingforserotoninbutnotmelatoninbiosynthesis(Martinezetal.,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
HaycockJW,KumerSC,LewisDA,VranaKE,StockmeierCA(2002)Amonoclonalantibodytotryptophanhydroxylase:applicationsandidentificationoftheepitope.JNeurosciMethods114:205-212.PMID:17727633
