Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Mostmammals
- Applications:
- WB1:10,000IHC1:1,000
- Species:
- Mouse
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2020-TUB,RRID:AB_2492275
Antigen/Purification:Collapse
TheantigenisasyntheticpeptidecorrespondingtobetaIIItubulin.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Tubulinisthemajorconstituentofmicrotubules,existingasaheterodimeroftheαandβsubunits.ThebetaIIIisoformoftubulinisfoundalmostexclusivelyinneuronalprocessesofadulttissuesandisthereforeanexcellentMarkerforneurons.Neuronspecific,posttranslationalmodificationswithintheC-terminaldomainofbetaIIItubulinhavebeenshowntobedevelopmentallyregulatedsuggestingthattheymayservetomodulatetheinteractionoftubulinwithmicrotubuleassociatedproteins(Leeetal.,1990).Additionally,betaIIItubulinhasbeenfoundtobehighlyexpressedincancercellssuchassmallcelllungcancer,largecellneuroendocrinecarcinomaandadenocarcinomasandiscorrelatedwithanincreasinghistologicaldegreeofmalignancy(Katsetosetal.,2003)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
ProductSpecificReferences
Ka,M.,&Kim,W.Y.(2015).Microtubule-ActinCrosslinkingFactor1IsRequiredforDendriticArborizationandAxonOutgrowthintheDevelopingBrain.Molecularneurobiology,November,1-15.PMID:26526844.
Cooper,R.J.,&Spitzer,N.(2015).SilvernanoparticlesatsublethalconcentrationsdisruptCytoskeletonandneuritedynamicsinculturedadultneuralstemcells.Neurotoxicology,48,231-238.PMID:25952507.
