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Functional_antibody >
PhosphoSolutions/Amyloid Precursor Protein Antibody/144-APP/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~115kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#144-APP,RRID:AB_2492039
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratAPP.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationonacolumnmadewiththeC-terminalpeptideusedasantigen.
BIOLOGicalSignificance:Collapse
Alargebodyofevidencehasimplicatedtheamyloidprecursorprotein(APP)inthepathogenesisofAlzheimer’sdisease(AD)(Kamenetzetal.,2003).ThephosphorylationofAPPatThr668isthoughttoplayacriticalroleingenerationofthesolubleAPP(beta)andbeta-amyloidpeptide(abeta)whicharethemajorcomponentsofsenileplaquesinpatientbrainsinflictedwithAD(Liuetal.,2003;Andoetal.,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences