Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Neatserum
- Specificity:
- Rat,Human
- Applications:
- WB1:1,000IHC1:100
- Species:
- Rabbit
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#592-FRAC,RRID:AB_2492097
Antigen/Purification:Collapse
TheantigenisaSyntheticpeptidetakenfromtheCterminusofthe~32kDaactinfragment.
Theantibodyisneatserum.
BIOLOGicalSignificance:Collapse
Fractin(fragmentofactin)isacaspase-specificcleavageproductofactinandservesasanovelMarkerofapoptosis-relatedevents.Theantibodyhasbeenshowntodetecttheprocessesandcellbodiesofdegeneratingneuronsandplaque-associatedmicrogliainAlzheimer’sdisease(Yangetal.,1998).IthasrecentlybeenreportedthatFractinmayhaveafunctionalroleinapoptoticsignalinginoligodendrocytes(Schulz,R.,etal.,Glia,2009,inpress).
Storage
Liquid.Containsnopreservative.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforuptooneyearafterdateofreceipt.Avoidfreeze/thawcycles.
ProductSpecificReferences
Yang,F,etal.,AntibodytoCaspase-CleavedActinDetectsApoptosisinDifferentiatedNeuroblastomaandPlaque-AssociatedNeuronsandMicrogliainAlzheimer’sdisease.AmericanJournalofPathology,1998,Vol.152,No.2,p.379-389.PMID:9466564
Rossiter,JP,etal.,Caspase-cleavedactin(fractin)immunolabelingofHiranobodies.NeuropatholApplNeurobiol.2000Aug;26(4):342-6. PMID:10931367
Asamec,E,etal.,Multiple-labelimmunocytochemistryfortheevaluationofnatureofcelldeathinexperimentalmodelsofneurodegeneration.BrainResProtoc.2001Jul;7(3):193-202.PMID:11431120
Chen,TA,etal.,Inhibitionofcaspase-3-likeactivityreducesglutamateinducedcelldeathinadultratretina.BrainRes.2001Jun15;904(1):177-88.PMID:11516428
Rossiter,JP,etal.,Caspase-3activationandcaspase-likeproteolyticactivityinhumanperinatalhypoxic-ischemicbraininjury.ActaNeuropathol.2002Jan;103(1):66-73.PMID:11841033
