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PhosphoSolutions/Methyl-CpG Binding Protein 2 (MeCP2) Antibody/1205-MeCP2/100 µl
Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGpurified
- Specificity:
- Human,Mouse,Rat
- Applications:
- WB1:2,000IF1:1,000IHC1:1,000
- Species:
- Mouse
- MolecularReference:
- ~75kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1205-MeCP2,RRID:AB_2632401
Antigen/Purification:Collapse
TheantigenisrecombinantfulllengthhumanMeCP2expressedinandpurifiedfromE.Coli.
TheantibodyisproteinGpurifiedfromculturesupernatant.
BIOLOGicalSignificance:Collapse
MeCP2(Methyl-CpGBindingProtein2)isachromosomalproteinthatbindstomethylatedDNA. Itcanbindspecificallytoasinglemethyl-CpGpairandisnotinfluencedbysequencesflankingthemethyl-CpGs. MeCP2hasbeenshowntomediatetranscriptionalrepressionthroughinteractionwithhistonedeacetylaseandthecorepressorSIN3A(Nanetal.,1998). DefectsinMeCP2arethecauseofRettsyndrome(RTT)(Amiretal.,1999). RTTisanX-linkeddominantdisease;itisaprogressiveneurologicdevelopmentaldisorderandoneofthemostcommoncausesofmentalretardationinfemales.
Storage
100µlinPBS+50%glycerol+5mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
AmirRE, VandenVeyverIB, WanM, TranCQ, FranckeU, ZoghbiHY (1999)RettsyndromeiscausedbymutationsinX-linkedMECP2,encodingmethyl-CpG-bindingprotein2. NatGenet. Oct;23(2):185-8.
NanX, NgHH, JohnsonCA, LahertyCD, TurnerBM, EisenmanRN, BirdA (1998)Transcriptionalrepressionbythemethyl-CpG-bindingproteinMeCP2involvesahistonedeacetylasecomplex. Nature. May28;393(6683):386-9.
