Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Human,Bovine,Non-humanprimate
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~52kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p197-101,RRID:AB_2492202
Antigen/Purification:Collapse
Theantigenisa Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser101ofhumanparkin.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
ParkinisanE3ligaseintheubiquitin-proteasomesystem.HereditaryParkinson’sdiseaseismostcommonlycausedbymutationsintheparkingeneandischaracterizedbytheprogressivelossofdopaminergicneuronsandthepresenceofLewybodiesinthesubstanianigra(Jenneretal.,1992).RecentevidencesuggeststhatphosphorylationofparkinatSer101mayhaveanimportantregulatoryroleonitsE3ubiquitinligaseactivity(Yamamotoetal.,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
RubiodelaTorreE,Luzón-ToroB,Forte-LagoI,Minguez-CastellanosA,FerrerI,HilfikerS.(2009)Combinedkinaseinhibitionmodulatesparkininactivation.HumMolGenet.Mar1;18(5):809-23.PMID:19050041
