Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~46kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1110-298,RRID:AB_2492086
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoTyr298ofXenopusEphrinB.Note:XenopusTyr298isthehomologofhuman,mouseandratTyr317andalsochickenTyr305.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
EphrinBproteinsarethoughttoplaykeyrolesincellularfunctionsasdiverseasneuronalmigrationandbloodvesseldevelopment(FlanaganandVancerhaeghen,1998;Dufouretal.,2003;Oikeetal.,2002).EphrinBmoleculesexpressedatthemembranesurfacebindtotheEphBfamilyreceptorsontargetcellsduringcelltocellcontact.ThisinteractionleadstocellsignalinginthetargetcellbutalsogeneratesareversesignalinthecellexpressingEphrinBonitssurface.Thisreversesignalingeventisthoughttobecriticalforvesselmaturationandneuronaldevelopment.Importantly,tyrosinephosphorylationofEphrinBisthoughttobeacriticalcomponentofthisreversesignalingevent(Palmeretal.,2002).RecentworksuggeststhatphosphorylationofaspecificEphrinBresidue(Tyr298)playsakeyroleinEphrinBsignaling(Kalo,etal.,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Bong,Y.S.,Park,Y.H.,Lee,H.S.,Mood,K.,Ishimura,A.andDaar,I.O.Tyr-298inephrinB1iscriticalforaninteractionwiththeGrb4adaptorprotein,Biochem.J.377:499-507(2004).
Dufour,A.,Seibt,J.,Passante,L.,Depaepe,V.,Ciossek,T.,Frisen,J.,Kullander,K.,Flanagan,J.G.,Polleux,F.andVanderhaeghen,P.Areaspecificityandtopographyofthalamocorticalprojectionsarecontrolledbyephrin/Ephgenes,Neuron39:453-465(2003).
Flanagan,J.G.andVanderhaeghen,P.TheephrinsandEphreceptorsinneuraldevelopment,Annu.Rev.Neurosci.21:309-345(1998).
Flanagan,J.G.andVanderhaeghen,P.TheephrinsandEphreceptorsinneuraldevelopment,Annu.Rev.Neurosci.21:309-345(1998).
Oike,Y.,Ito,Y.,Hamada,K.,Zhang,X.Q.,Miyata,K.,Arai,F.,Inada,T.,Araki,K.,Nakagata,N.,Takeya,M.,Kisanuki,Y.Y.,Yanagisawa,M.,Gale,N.W.andSuda,T,RegulationofvasculogenesisandangiogenesisbyEphB/ephrin-B2signalingbetweenendothelialcellsandsurroundingmesenchymalcells,Blood100:1326-1333(2002).
Palmer,A.,Zimmer,M.,Erdmann,K.S.,Eulenburg,V.,Porthin,A.,Heumann,R.,Deutsch,U.andKlein,REphrinBphosphorylationandreversesignaling:regulationbySrckinasesandPTP-BLPhosphatase,MolCell9:725-737(2002)
