Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~46kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1110-317,RRID:AB_2492087
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoTyr317ofChickenEphrinB.Note:ChickenTyr317 isthehomologofratandmouseTyr328,humanTyr329andXenopusTyr310.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
EphrinBproteinsarethoughttoplaykeyrolesincellularfunctionsasdiverseasneuronalmigrationandbloodvesseldevelopment(FlanaganandVanderhaeghen,1998;Dufouretal.,2003;Oikeetal.,2002).EphrinBmoleculesexpressedatthemembranesurfacebindtotheEphBfamilyreceptorsontargetcellsduringcell-tocellcontact.ThisinteractionleadstocellsignalinginthetargetcellbutalsogeneratesareversesignalinthecellexpressingEphrinBonitssurface.Thisreversesignalingeventisthoughttobecriticalforvesselmaturationandneuronaldevelopment.Importantly,tyrosinephosphorylationofEphrinBisthoughttobeacriticalcomponentofthisreversesignalingevent(Palmeretal.,2002).RecentworksuggeststhatphosphorylationofaspecificEphrinBresidue(Tyr298)playsakeyroleinEphrinBsignaling(Kalo,etal.,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
WeirongXing,JonghyunKim,JonWergedal,Shin-TaiChen,andSubburamanMohan(2010)EphrinB1RegulatesBoneMarrowStromalCellDifferentiationandBoneFormationbyInfluencingTAZTransactivationviaComplexFormationwithNHERF1.Mol.Cell.Biol.,30:711–721.PMID:19995908
