Chickenpolyclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgYfraction
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:2,000IF1:500
- Species:
- Chicken
- MolecularReference:
- ~43kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#875-GAP43,RRID:AB_2492122
Antigen/Purification:Collapse
TheantigenisasyntheticpeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionoftheratGAP43sequence.
TheantibodyistotalIgYfraction.
BIOLOGicalSignificance:Collapse
GAP-43isthoughttohaveanimportantroleindevelopmentandplasticitybecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandduringaxonalregeneration(BenowitzandRouttenberg,1997).ThereisalsoevidencefromknockoutanimalsthatGAP-43servestoamplifypathfindingsignalsfromthegrowthcone(Strittmatteretal.,1995).GAP-43isthoughttomediateatleastsomeoftheseeffectsviainteractionwithactin.Importantly,phosphorylationatSer41byproteinkinaseCmodulatestheinteractionofGAP-43withactin(Heetal.,1997)andmayalsoaffectneurotransmitterreleaseduringformsofplasticitylikeLTP(Huloetal.,2002).
Storage
TotalIgYfractioninPBS+10mMNaN3.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BenowitzLI,RouttenbergA(1997)Gap-43:Anintrinsicdeterminantofneuronaldevelopmentandplasticit.TrendsNeurosci20:84-91.
He,Q,Dent,EW,Meiri,KF(1997)ModulationofactinfilamentbehaviorbyGap-43(neuromodulin)isdependentonthephosphorylationstatusofserine41,theproteinkinaseCsite.JNeurosci17:3515-3524.
HuloS,Alberi,S,LauxT,MullerD,CaroniP(2002)ApointmutantofGap-43inducesenhancedshort-termandlongtermhippocampalplasticity.EurJNeurosci15:1976-1982.
StrittmatterSM,FankhauserC,HuangPL,MashimoH,FishmanMC(1995)NeuronalpathfindingisabnormalinmicelackingtheneuronalgrowthconeproteinGap-43,”Cell80:445-452.
RayuduGopalakrishna,UshaGundimeda,JasonEricSchiffman,andThomasH.McNeill(2008)ADirectRedoxRegulationofProteinKinaseCIsoenzymesMediatesOxidant-inducedNeuritogenesisinPC12CellsJ.Biol.Chem.,May2008;283:14430–14444.
