Rabbit polyclonal antibody
Pooled Serum- Size:
- 100 µl
- Formulation:
- Affinity Purified from Pooled Serum
- Specificity:
- Bovine, Chicken, Guinea Pig, Human, Mouse, Non-human primate, Rat, Sheep
- Applications:
- WB 1:1000
- Species:
- Rabbit
- Molecular Reference:
- ~ 51 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1208-222, RRID:AB_2572427
Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser222 of the human MEF2C protein.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: Collapse
MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015). Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015). Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015). Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Brown, F., Still, E., Cifani, P., Takao, S., Reed, C., Ficarro, S., Koche, R., Romanienko, P., Mark, W., O’Donnell, C., Spitzer, B., Stutzke C., Krivtsov, A., Pouliot, G., Gray, N., Marto, J., Armstrong, S., and Kentsis, A. 2017. MEF2C phosphorylation is required for chemotherapy resistance in acute myeloid leukemia. bioRxiv, p.107201.
