Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Zebrafish,GuineaPig
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~32kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1540-1013,RRID:AB_2492194
Antigen/Purification:Collapse
Theantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser10,13,14ofOlig2.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
LigonKL,AlbertaJA,KhoAT,WeissJ,KwaanMR,NuttCL,LouisDN,StilesCD,RowitchDH.TheoligodendrogliallineageMarkerOLIG2isuniversallyexpressedindiffusegliomas.JNeuropatholExpNeurol.2004;63:499–509.
LigonKL,HuillardE,MehtaS,KesariS,LiuH,AlbertaJA,BachooRM,KaneM,LouisDN,DepinhoRA,AndersonDJ,StilesCD,RowitchDH.(2007)Olig2-regulatedlineage-restrictedpathwaycontrolsreplicationcompetenceinneuralstemcellsandmalignantglioma.Neuron.2007;53:503–17.
SunY,MeijerDH,AlbertaJA,MehtaS,KaneMF,TienAC,FuH,PetryniakMA,PotterGB,LiuZ,PowersJF,RunquistIS,RowitchDH,StilesCD.(2011)PhosphorylationstateofOlig2regulatesproliferationofneuralProgenitors.Neuron69(5):906-17
