Sheeppolyclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGpurified
- Specificity:
- Rat,Mouse,Human,Non-humanprimate
- Applications:
- WB1:1,000IF1:500IHC1:500
- Species:
- Sheep
- MolecularReference:
- ~29kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#303-CAL,RRID:AB_2492050
Antigen/Purification:Collapse
Theantigenisa purifiedguineapigcalretinin.
TheantibodyisproteinApurifiedsheepserum.
BIOLOGicalSignificance:Collapse
Calretininisacalcium-bindingproteininvolvedincalciumsignaling.Itisabundantlyexpressedthroughoutthecentralnervoussystem,retinaandmanyothertissues.Studieshaveshownthatcalretininmayplayaroleinneuroprotectionbybufferingintracellularcalcium(LukasandJones1994,Weisenhornetal.,1996).Additionally,calretininselectivelystainsmesothelialcellsthusmakingitanimportantMarkerfordifferentiatingmalignantmesotheliomafromcarcinomas(Attanoosetal.,2000).
Storage
100µlin10mMTris,100mMNaCl.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
AttanoosRL,DojcinovSD,WebbR,GibbsAR.(2000)Anti-mesothelialmarkersinsarcomatoidmesotheliomaandotherspindlecellneoplasms.Histopathology.37(3):224-31.
IsaacsKR,WinskyL,StraussKI,JacobowitzDM.(1995)Quadruplecolocalizationofcalretinin,calcitoningene-relatedpeptide,vasoactiveintestinalpeptide,andsubstancePinfiberswithinthevillioftheratintestine.CellTissueRes.,280(3):639-51.
LukasW,JonesKA(1994)Corticalneuronscontainingcalretininareselectivelyresistanttocalciumoverloadandexcitotoxicityinvitro.Neurosci.61(2):307-16.
WeisenhornDMV,Weruaga-PrietoE,CelioMR(1996)Calretininimmunoreactivityinorganotypicculturesoftheratcerebralcortex:effectsofserumdeprivation.Exp.BrainRes.108(1):101-112.
