Goatpolyclonalantibody
- Size:
- 100µl
- Formulation:
- AffinityPurified
- Specificity:
- Rat,Mouse,Human,Chicken,Non-humanprimate,GuineaPig
- Applications:
- WB1:100IHC1:100
- Species:
- Goat
- MolecularReference:
- ~70kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#315-CHAT,RRID:AB_2492055
Antigen/Purification:Collapse
Theantigenisanativecholineacetyltransferasepurifiedfromhumanplacenta.
Theantibodyispreparedfromgoatserumbyaffinitypurificationusingacolumntowhichtheproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
CholineacetyltransferaseisaneuronalenzymewhichcatalyzesthereactionbetweenAcetylCoAandcholineresultingintheformationofacetylcholine.ItisthereforefoundprimarilyincholinergicneuronsmakingitavaluableMarkerfordiseasesassociatedwithdecreasedcholinergicfunctionsuchasSchizophrenia,Alzheimer’sdisease(AD)andDownsyndrome(Holtetal.1999).DecreasedcholineacetyltransferaseactivityinparticularhasbeenshowninSchizophrenicsubjects(Karsonetal1993).IthasfurThermorebeendemonstratedthatinpatientswithAD,therearesignificantlylowerlevelsofcorticalChATthatcorrelatewithseverityofthediseaseasmeasuredbylossofneuropsychologicalfunction(Baskinetal.1999).
Storage
100µlinPBS+5mg/mlBSA+0.2%NaN3.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
KarsonCN,CasanovaMF,KleinmanJE,GriffinWS(1993)Cholineacetyltransferaseinschizophrenia.Am.J.Psychiatry150:454-459.
HoltDJ,HermanMM,HydeTM,KleinmanJE,CintonCM,GermanDC,HershLB,GreybielAM,SaperCB(1999)Evidenceforadeficitincholinergicinterneuronsinthestriatuminschizophrenia.Neuroscience94(1):21-31.
BaskinDS,BrowningJL,PirozzoloFJ,KorporaalS,BaskinJA,AppelSH(1999)BraincholineacetyltransferaseandmentalfunctioninAlzheimerdisease.ArchNeurol.56:1121-1123.
