Rabbitpolyclonalantibody
PooledSerum- Size:
- 200µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~48kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#700-GLY,RRID:AB_2492133
Antigen/Purification:Collapse
TheantigenisapeptidefromtheN-terminusregionoftheα1-subunitofratglycinereceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Glycineisanimportantinhibitorytransmitterinthebrainstemandspinalcord.Glycinereceptorsaremembersoftheligand-gatedionchannelfamily(LGICs)thatmediaterapidchemicalneurotransmission(Schofieldetal.,2003).Thebindingofglycinetoitsreceptorproducesalargeincreaseinchlorideconductance,whichcausesmembranehyperpolarization.Glycinereceptorsareanchoredatinhibitorychemicalsynapsesbyacytoplasmicprotein,gephyrin(Fischeretal.,2000).TheglycinereceptorhasbeenusedtogreatadvantageintheidentificationofthebindingsitesforalcoholontheLGICfamilyofproteins(Becksteadetal.,2001;Mihicetal.,1997).Thesereceptorshavealsobeenextremelyusefulinstudiesofsynapticclusteringofreceptors(CraigandLichtman,2001).TheglycinereceptormayalsoactinconcertwithanNMDARsubunittoformanexcitatoryreceptor(Chattertonetal.,2002).
Storage
200µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
WickMJ,BleckV,WhatleyVJ,BrozowskiSJ,NixonK,CardosoRA,andValenzuelaCF(1999)Stableexpressionofhumanglycinealpha1andalpha2homomericreceptorsinmouseL(tk-)cells.JNeurosciMethods87(1):97-103.PMID:10065988
