RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Rat
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~52kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1470-nACB2,RRID:AB_2619712
Antigen/Purification:Collapse
Theantigenisafusionproteinfromthecytoplasmicloopoftheβ2-subunitofratnAChR.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Nicotinicacetylcholinereceptors(nAChRs)areionotropic,cholinergicreceptorsthataredividedinto2types;muscletypeandneuronaltype.NeuronalnAChRsarepentamericionchannelsconsistingof5identical(homopentamers)ordifferent(heteropentamers)subunits.HeteropentamericneuronalnAChRsmediatefastsynaptictransmissionintheautonomicnervoussystem.Thepredominanthetero-oligomericnAChRintheCNScontainthesubunitsα4β2,whereasα3β4prevailinthePNS.However,theexpressionofthesesubunitsvariesnotonlybyregionbutalsoduringdevelopment(Scholzeetal2011).Inthebrain,β2-containingreceptorsgreatlyoutnumberreceptorsthatcontainβ4(McGehee&Role,1995;Albuquerqueetal.,2009),andinmostbrainregions,targeteddeletionoftheβ2subunitvirtuallyabolishes[3H]-epibatidinebindingandreceptorautorADIography(Zolietal.,1998)duetotheabsenceofaβsubunitrequiredtoformfunctionalnAChRs(Champtiaux&Changeux,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Scholze,P.,Ciuraszkiewicz,A.,Groessl,F.,Orr‐Urtreger,A.,McIntosh,J.M.,&Huck,S.(2011).α4β2nicotinicacetylcholinereceptorsintheearlypostnatalmousesuperiorcervicalganglion.Developmentalneurobiology,71(5),390-399.
McGehee,D.S.,&Role,L.W.(1995).Physiologicaldiversityofnicotinicacetylcholinereceptorsexpressedbyvertebrateneurons.Annualreviewofphysiology,57(1),521-546.
Albuquerque,E.X.,Pereira,E.F.,Alkondon,M.,&Rogers,S.W.(2009).Mammaliannicotinicacetylcholinereceptors:fromstructuretofunction.Physiologicalreviews,89(1),73-120.
Zoli,M.,Léna,C.,Picciotto,M.R.,&Changeux,J.P.(1998).Identificationoffourclassesofbrainnicotinicreceptorsusingβ2mutantmice.TheJournalofneuroscience,18(12),4461-4472.
Champtiaux,N.,&Changeux,J.P.(2004).Knockoutandknockinmicetoinvestigatetheroleofnicotinicreceptorsinthecentralnervoussystem.Progressinbrainresearch,145,233-251.
