Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~100kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1550-503,RRID:AB_2492212
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser503ofthevoltage-gatedpotassiumchannelKv3.1,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Voltage-gatedK+channelsareimportantdeterminantsofneuronalmembraneexcitABIlity.Moreover,differencesinK+channelexpressionpatternsanddensitiescontributetothevariationsinactionpotentialwaveformsandrepetitivefiringpatternsevidentindifferentneuronalcelltypes(Maletic-Savaticetal.,1995;Pongs,1999;BlaineandRibera,1998;BurgerandRibera,1996).TheKv3.1potassiumchannelisexpressedathighlevelsinneuronsthatcharacteristicallyfirerapidtrainsofactionpotentials(Ganetal.,1999).Particularlyhighlevelsofthischannelarefoundinneuronsoftheauditorybrainstem.Theseneuronsappeartoparticipateinneuralcircuitsthatdeterminetheintensityandtimingofauditorystimuliandusethisinformationtodeterminethelocationofsoundsinspace(vonHehnetal.,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Product SpecificReferences
PingSongandLeonardK.Kaczmarek(2006)ModulationofKv3.1bPotassiumChannelPhosphorylationinAuditoryNeuronsbyConventionalandNovelProteinKinaseCIsozymesJ.Biol.Chem.,Jun2006;281:15582-15591.PMID:16595659
