Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Sheep,Canine
- Applications:
- WB1:250
- Species:
- Rabbit
- GeneName:
- BECN1
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p117-234,RRID:AB_2492047
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser234ofhumanbeclin-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Beclin-1playsakeyroleintumorsuppressionandautophagy.Ithasbeensuggestedthatautophagyproteinssuchasbeclin-1caninhibittumOrigenesisandthatdecreasedexpressionoftheseproteinsmaycontributetothedevelopmentofhumanmalignancies(Liangetal.,1999).Beclin-1hasbeenshowntointeractwith14-3-3proteinsthroughAkt-mediatedphosphorylationatSer234(Wangetal.,2012).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
RichardC.Wang,YongjieWei,ZhenyiAn,ZhongjuZou,GuanghuaXiao,GovindBhagat,MichaelWhite,JuliaReichelt,andBethLevine(2012)Akt-MediatedRegulationofAutophagyandTumorigenesisThroughBeclin1Phosphorylation.Science338:956-959.PMID:23112296
