Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Chicken,Non-humanprimate,Sheep
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p153-358,RRID:AB_2492084
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser358ofhumaneEF1A2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
EukaryoticElongationFactoreEF1Aexistsintwovariantforms,eEF1A1andeEF1A2.WhileeEF1A1isalmostubiquitouslyexpressedinhumans,eEF1A2ispredominantlyfoundinheart,brain,andskeletalmuscle(Knudsenetal.,1993).ExpressionofeEF1A2mayhavearoleinovariancancer,asitsexpressionisdrasticallyincreasedinhumanovariantumors(Anandetal.,2002).Duetodifferencesinstructuralmodelsbetweenthetwoisoforms,eEF1A1andeEF1A2likelyhavevariant-specificphosphorylationsites(Soaresetal.,2009).Ribosome-associatedJNKphosphorylatesSer358oneEF1A2topromotedegradationofnewlysynthesizedpolypeptidesbytheproteasome(Gandinetal.,2013).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Gandin,V.,Gutierrez,G.J.,Brill,L.M.,Varsano,T.,Feng,Y.,Aza-Blanc,P.,Au,Q.,McLaughlan,S.,Ferreira,T.A.,Alain,T.,Sonenberg,N., TopisirovicI,&RonaiZA(2013).Degradationofnewlysynthesizedpolypeptidesbyribosome-associatedRACK1/c-JunN-terminalkinase/eukaryoticelongationfactor1A2complex.Molecularandcellularbiology,33(13),2510-2526.PMID:23608534
