Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~46kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p155-383,RRID:AB_2492085
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser383ofhumanElk-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Elk-1isatranscriptionfactorinvolvedinmediatinggenetranscriptioninresponsetogrowthfactors(HillandTreisman,1995).Elk-1isthoughttobephosphorylatedbyMAPkinaseatSer383andphosphorylationatthissiteisessentialforthetranscriptionalactivityofElk-1(Lietal.,2003).PhosphorylationofElk-1hasalsobeenimplicatedinsynapticplasticityintheadulthippocampus(Thielsetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
HillCS,TreismanR(1995)Transcriptionalregulationbyextracellularsignals:mechanismsandspecificity.Cell80:199-211.
LiQJ,YangSH,MaedaY,SladekFM,SharrocksAD.Martins-GreenM(2003)MAPkinasephosphorylationdependentactivationofElk-1leadstoactivationoftheco-activatorp300.EMBOJ22:281-291.
ThielsE,KanterewiczBI,NormanED,Trzaskos,JM,KlannE(2002)Long-termdepressionintheadulthippocampusinvivoinvolvesactivationofextracellularsignal-regulatedkinaseandphosphorylationofElk-1.JNeurosci22:2054-2062.
