Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Human,Non-humanprimate
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~83kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p170-679,RRID:AB_2492091
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser679/683ofthehumanFAM129Bsequence.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
FAM129B,alsoknownasNiban-likeprotein1,belongstoapoorlycharacterizedproteinfamilywithunknowncategoryandfunction.IncreasedexpressionoftheNibangenehasbeenobservedinrenalcarcinomas(Adachietal.,2004;Sunetal.,2007).SuppressionofFAM129BexpressioninHeLacellshasbeenseentopromoteapoptosis,suggestingthatitcanmodulatecelldeathsignaling,andmaybeinvolvedintheERstressresponse(Sunetal.,2007).FAM129Bisalsoup-regulatedinvarioustypesofthyroidtumorsandHashimoto’sthyroiditis(Matsumotoetal.,2006).IthasbeensuggestedthattheMAPkinasedependentphosphorylationofFAM129Bisimportantincontrollingmelanomacells,asinhibitionofB/Raf/MKK/ERKinmelanomacellsrepressesinvasion(Oldetal.,2009).ItisbelievedthatphosphorylatedFAM129Bnotonlyderepressesinvasion,butalsoregulateseventsthatpromoteinvasion(Oldetal.,2009).
Storage
Liquidin100mMGlycine,200mMTris,pH8.0.Containsnopreservative.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
AdachiH,MajimaS,KonS,KobayashiT,KajinoK,MitaniH,HirayamaY,ShiinaH,IgawaM,HinoO.Nibangeneiscommonlyexpressedintherenaltumors:anewcandidateMarkerforrenalcarcinogenesis.Oncogene.2004Apr22;23(19):3495-500.
SunGD,KobayashiT,AbeM,TadaN,AdachiH,SiotaA,Totsuka,HinoO.Theendoplasmicreticulumstress-inducIBLeproteinNibanregulateseIF2alphaandS6K1/4E-BP1phosphorylation.BiochemBiophysResCommun.2007Aug17;360(1):181-7.
MatsumotoF,FujiiH,AbeM,KajinoK,KobayashiT,MatsumotoT,IkedaK,HinoO.Anoveltumormarker,Niban,isexpressedinsubsetsofthyroidtumorsandHashimoto’sthyroiditis.HumanPathology.2006Dec;37(12):1592-600.
OldW,ShabbJ,HouelS,WangH,CoutsK,YenC,LitmanE,CroyC,Meyer-ArendtK,MirandaJ,BrownR,WitzeE,SchweppeR,ResingK,AhnN.FunctionalProteomicsIdentifiesTargetofPhosphorylationbyB-RafSignalinginMelanoma.MolecularCell.2009Apr10(34):115-131
