Rabbit polyclonal antibody
Pooled Serum- Size:
- 100 µl
- Formulation:
- Affinity Purified from Pooled Serum
- Specificity:
- Rat, Mouse, Human
- Applications:
- WB 1:1,000
- Species:
- Rabbit
- Molecular Reference:
- ~53 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p195-392, RRID:AB_2492196
Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser392 of human p53.
The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002; Yin et al., 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser392 in p53 is associated with formation of human tumors (Saito et al., 2003; Pise-Masison et al., 1998; Kim et al., 2004). In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998)
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
General References
Jiang YH, Armstrong D, Albrecht U, Atkins CM, Noebels JL , Eichele G, Sweatt JD, Beaudet AL (1998) Mutation of the angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation. Neuron 21:799-811.
Kim YY, Park BJ, Kim DJ, Kim WH, Kim S, Oh KS, Lim JY, Kim J, Park C, Park SI (2004) Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability. FEBS Lett 572:92-98.
Lane DP (1992) p53, guardian of the genome. Nature (London) 358:15-16.
Pise-Masison CA, Radonovich M, Sakaguchi K, Appella E, Brady JN (1998) Phosphorylation of p53: a novel pathway for p53 inactivation in human T- cell lymphotropic virus type 1-transformed cells. J Virol 72:6348-6355.
Saito S, Yamaguchi H, Higashimoto Y, Chao C, Xu Y, Fornace AJ, Jr., Appella E, Anderson CW (2003) Phosphorylation site interdependence of human p53 post-trans lational modifications in response to stress. J Biol Chem 278:37536-37544.
Sharpless NE, DePinho RA (2002) p53: Good cop/bad cop. Cell 110:9-12.
Yin Y, Tainsky MA, Bischoff FZ, Strong LC, Wahl GM (1992) Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles. Cell 70:937-948.
